Last week, Information minister Jim Muhwezi banned the live coverage of FDC activities related to the defiance campaign.
Government said if opposition felt pushed to the wall, they could appeal in courts of law.
Winnie Byanyima who has been monitored by police throughout the weekend says the prayers are to be held Monday afternoon.
The aim of the prayers is to ensure Kizza Besigye is released from house arrest under which he was placed a week ago as part of the ban on opposition activities.
Winnie in her public appeal to all Ugandans, urges citizens to gather and pray for the future of the country and for her husband’s release from what she termed as “illegal detention”.
“Supporters in Kampala are asked to attend services at one of four locations: Church of the Resurrection Bugolobi, Kamwokya Church of God, Nakasero Mosque or Kibuli Mosque.”
She said all faiths and denominations are welcome at all locations.
“This is a time for all Ugandans to dedicate themselves to one another and to the collective betterment of our country.”
Winnie asked those outside Kampala are asked to join with friends and neighbours in fellowship at any convenient place, public or private, at the noon hour.
“Please share images or descriptions of these gatherings with the country on social media under the hashtag ”Free my vote” if possible.”
According to her, Uganda has been in political crisis since February’s discredited elections, which the incumbent president, Yoweri Museveni, is attempting to use to justify his continued his rule.
The opposition and civil society, led by Dr. Kizza Besigye, has asked for an independent audit of the electoral process.
Dr. Besigye is presently being detained without charge in his home by Ugandan security forces.